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SRX7389895: Bacillus Transcriptome analysis
1 ILLUMINA (Illumina HiSeq 4000) run: 5.7M spots, 1.1G bases, 361.4Mb downloads

Design: After removing the rRNA, the total RNA was interrupted to short fragments and the first-strand cDNA was synthesized with random hexamer-primer. Then the second-strand cDNA was synthesized with dATPs,dGTPs,dCTPs,dUTPs, RNase H and DNA polymerase I. The obtained fragments were purified with QiaQuick PCR extraction kit, repaired the end, added poly(A), connected the sequencing adapters and degraded the second-strand cDNA with UNG enzyme. Finally, the resulting fragment was purified by MiniElute PCR Purification Kit before PCR amplification.
Submitted by: Huaiyin Institute of Technology
Study: Bacillus Transcriptome or Gene expression
PRJNA510544 • SRP237815 • All experiments • All runs
show Abstracthide Abstract
Transcriptome Sequencing of 9 Bacillus (Bs916) and Mutants
Sample:
SAMN10791846 • SRS5838528 • All experiments • All runs
Organism: Bacillus subtilis
Library:
Name: BsH2
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RT-PCR
Layout: PAIRED
Runs: 1 run, 5.7M spots, 1.1G bases, 361.4Mb
Run# of Spots# of BasesSizePublished
SRR107095275,709,1451.1G361.4Mb2019-12-17

ID:
9675190

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